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Welcome to the Imaris 8.3 release notes. Please have a look to the overview of Imaris Release notes for information about prior released features and fixed bugs.

Version Date: May 27, 2016

Membrane Based Cell Boundary Detection

Using Imaris’ Cell component it is now possible to detect cell boundaries based on a membrane signal even if no nucleus signal is available. The following screenshots show examples of this segmentation:

Algorithmically Imaris performs a multi-scale watershed with region merging to achieve this segmentation. Users can interactively adjust the merging thresholds to get an immediate visual effect of the threshold settings. In addition after running the membrane based cell boundary detection users can go to the edit tab of Cells and Merge or Split cells that were not segmented correctly.

Cells Visualization Improved

Improved visualization of Cells on 2D slicers facilitate easier validation of segmentation results. New features include:

Border rendering of cells as white lines

Region rendering of cells with original intensities overlayed by cell color

Cells Color Options

Cells can now be colored by ObjectID to facilitate discrimination of neighbouring cells.

Lineage Tracking Algorithm “Maximum Overlap” for Cells

To track cells that are segmented from a membrane signal the “maximum overlap” tracking algorithm was added to Imaris8.3. This algorithm can track dividing cells to produce lineages.

The algorithm works as follows: For each cell the algorithm searches a mother cell at the previous time point by calculating the spatial overlap with all cells of the previous time point and picking as the mother that cell wit